Review Article
Biosynthesis and Role of N-Linked Glycosylation in Cell Surface Structures of Archaea with a Focus on Flagella and S Layers
Table 4
Effects of agl gene deletions on S layer glycosylation in H. volcanii.
| Gene deletion | Function | Glycan structure | Growth in high salt | MW shift of S layer | Susceptibility of S layer to proteases | Shedding of S layer | S layer symmetry |
| aglB | OTase | No glycan | Deficient | Yes | Same as WT | Increased | No effect | aglD | 5th GTase | Missing 5th sugar | Deficient | Yes | Less than WT | Decreased | Symmetry defect | aglE | 4th GTase | Missing 4th and 5th sugars | No effect | No | Same as WT | Same as WT | No effect | aglF | Glucose-1-P Uridyltransferase (sugar 3) | Mix of mono and disaccharides | No effect | Yes | More than WT | Same as WT | No effect | aglG | 2nd GTase | Missing last 4 sugars | No effect | Yes | More than WT | Same as WT | No effect | aglI | 3rd GTase | Mix of mono and disaccharides | No effect | Yes | More than WT | Same as WT | No effect | aglM | UDP-Glucose dehydrogenase (sugars 2 and 3) | Missing last 4 sugars | Not reported | Yes | More than WT | Not reported | Not reported | aglP | Methyl transferase (sugar 4) | Missing 5th sugar and methyl group of sugar 4 | Not reported | Not reported | More than WT | Not reported | Not reported |
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