Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi
Figure 3
Purified antibodies specific for the internal C1 Hsp70 protein does not inhibit E. cuniculi spore adherence to rabbit kidney host cells. Increasing concentrations of C1 antibodies (open circles) or EnP1 specific antibodies (open triangle) were incubated with spores in the presence of confluent host cells. Control samples excluded antibodies. After 4-hours, unbound spores were removed by washing and the bound spores were quantified by immunofluorescence assay. Each antibody dilution sample was performed in triplicate. Statistical significance is indicated with asterisks ().
