Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains

Table 3

Primers and probes designed from the reference sequences of the studied genes.

	Forward primer	Reverse primer	Probe	Genbank access
PGP A	GAGGGTGTGCAGATGCGGTA	CATGAACGTCAGCAGCAGCG	FAM- CTTCCCACTCCCTGTCCGCCCGA- TAMRA	X17154
MDR 1	GCGACCTGAACCTGACGATC	GCCCGATCATCGACGACTTG	FAM-CGCACCCAGACGACCCAGAGAACG- TAMRA	L01572
GSH	CTCCTTTGCGACACCGATGT	GAGTCGTAGCGAGACTTGAGAAT	FAM-CGGCACCTCCTCCAAGCGGCG- TAMRA	Y10049
DHFR	GTACCTTGAGCTGATTGACC	AGATGTGAATGGGGTCCTTGTCC	FAM-CTGCTGACGACGAAGCGTGT- TAMRA	AY122331
PTR 1	GCTGTCTGTTTGCACTATCA	CTTGAAGAGGGTAACAGGTG	FAM-TTGGCAACGTTGCTCAGGTC- TAMRA	AY547305
DNA POL	TGTCGCTTGCAGACCAGATG	GCATCGCAGGTGTGAGCAC	HEX-CCAGGCTCGAAGTTGTTGCTGCCC- TAMRA	AF009141
ASC PEROX	ATGATCTCTGAAAAGCTGGA	AGGGATATCGAGACCTTTGT	HEX-GCTTCAAACCGGAATGCCTG- TAMRA	XM 001686044