Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains

Table 4

Reference values (means, standard deviations, thresholds) from the sensitive reference strain and amplification coefficients of the clinical primo-isolates displaying amplification ratios above the threshold values calculated from the reference strain.

		PGP A	MDR 1	GSH	DHFR	PTR1
Reference strain (calibrator, 10 replicates)	Mean value	1.06	1.00	1.07	1.06	1.02
	Standard deviation	0.27	0.33	0.22	0.27	0.11
	Threshold value	1.87	1.99	1.73	1.87	1.35
Isolates displaying amplified genes	Minimum value	3.07	2.24	1.83	2.49	1.49
	Maximum value	22.2	62.8	2.48	16.05	2.7
	Mean value	8.42	6.98	2.41	10.6	1.85
	Number of isolates	5	30	3	5	3