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International Journal of Microbiology
Volume 2010, Article ID 826830, 6 pages
http://dx.doi.org/10.1155/2010/826830
Research Article

Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla

1Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800 Minden, Malaysia
2Phytochemistry laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang, 11800 Minden, Malaysia

Received 6 November 2009; Accepted 4 April 2010

Academic Editor: Giuseppe Comi

Copyright © 2010 Amir Modarresi Chahardehi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities from Pilea microphylla (Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at . Chloroform extract of method I (CE I) showed the highest total phenolic contents at and chloroform extract of method II (CE II) showed the highest total flavonoid contents at . The antimicrobial activity of Pilea microphylla extract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). The Pilea microphylla extract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 g/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.