Antioxidant, Antimicrobial Activity and Toxicity Test of Pilea microphylla

Table 1

List of different crude extract, depicting variable total phenolic content, total flavonoid content, antioxidant activity*, and EC₅₀ values.

Name of crude extract	Antioxidant activity (%)	EC50 values (𝜇g/mL)	Total phenolic (mg GAE/g dw)	Total flavonoid (mg QE/g)	Total phenolic/Total flavonoid
HE I	28.16±4.76^c	ND	11.35±0.26^ab	0.45±0.49^a	25.22
CE I	0.46±0.98^a	ND	72.10±0.71^g	53.44±0.94^e	1.35
EAE I	4.10±0.14^a	ND	41.82±1.34^e	11.57±1.07^c	3.61
ME I	69.51±1.03^f	81.32	9.91±0.33^a	ND	ND
ME II	46.99±4.69^d	121.90	13.76±1.52^b	ND	ND
CE II	47.16±4.98^d	215.30	35.02±0.11^d	60.14±0.33^f	0.58
DE II	60.69±2.46^e	373.50	54.48±1.65^f	21.84±1.65^d	2.49
EAE II	8.62±0.95^a	ND	40.76±2.22^e	7.58±0.23^b	5.38
BE II	18.34±2.34^b	ND	22.47±2.03^c	ND	ND

Each value represented the mean±SD of three replicates (𝑛=3). Values with different letters are significantly different(𝑃<05.)based on One Way and Tukey HSD test.
Data of total phenolic contents are expressed as milligrams of gallic acid (GAE) equivalents per microgram dry weight.
Quercetin and BHT were used as a positive control, EC₅₀= 4.95 𝜇g/mL and 50.31 𝜇g/mL and the final concentration were 125 𝜇g/mL.
ND = Not determined
∗Modarresi Chahardehi et al. [9]