International Journal of Microbiology

Research Article

Fluorogenic Membrane Overlays to Enumerate Total and Fecal Escherichia coli and Total Vibrionaceae in Shellfish and Seawater

Table 2

Other Enterobacteriaceae used in this study and the results of 4-methyl- -D-glucuronide (MUG) overlay for the detection of -glucuronidase-producing bacteria, most probable number (MPN) assay in EC tubes containing MUG, and colony overlay procedure for peptidases (COPP) overlay for lysyl aminopeptidase activity.
PathogenID codeSource1No. MUG+ in triplicate overlays2MPN-MUG tubes3COPP overlay2
GrowthGasMUG fluorescence
Citrobacter braakii43162ATCC0−−−−
Citrobacter freundii8090ATCC0−−−−
Citrobacter freundii33128USDA0−−−−
Klebsiella pneumonia13883ATCC0−−−−
Pseudomonas aeruginosa7700ATCC0−−−–
Pseudomonas fluorescens43892ATCC0−−−−
Salmonella Dublin15480ATCC0±−−−
Salmonella EnteritidisSAL 196 H 3502USDA0±−−−
Salmonella MontevideoSAL 2#61 FSIS 051USDA3±−+−
Salmonella NewportSAL1#86 H 1073USDA0±−−−
Salmonella TyphimuriumG 7601 WI U 302USDA0±−−−
Salmonella TyphimuriumH 3380 CA DT 104USDA0±−−−
Salmonella TyphimuriumSAL 2#46 FSIS 026USDA0±−−−
Serratia liquefaciens35551ATCC0−−−−
Shigella boydii9207ATCC2−−−−
Shigella boydiiBS512USUHS3±−++
Shigella dysenteriaeBS506USUHS0−−−+
Shigella flexneri2457TUSUHS0−−−−
Shigella flexneri12022ATCC0−−−−
Shigella sonneiBS514USUHS3±−+−
Shigella sonnei25931ATCC3±−+−
Yersinia enterocoliticaO:8, 12#08USDA0−−−+
1Abberviaitons: ATCC: American Type Culture Collection, Manassas, Virginia; USDA: United States Department of Agriculture, Agricultural Research Service, Wyndmoor, Pennsylvania; USUHS: Uniformed Services University of the Health Sciences, Bethesda, Maryland.
2MUG and COPP overlays were performed at .
3MPN-MUG assays were incubated at .