Research Article

Boric Acid Disturbs Cell Wall Synthesis in Saccharomyces cerevisiae

Figure 6

Slt2p-signaling in BA-treated cells. (a) Detection of phosphorylated Slt2p by western blotting in strain YPH499. Phosphorylation of Slt2p increases with escalating concentrations of BA. The total amount of Slt2p increases only slightly under the same condition. Gamma tubulin (Tub4p) detection by western blotting and the Coomassie blue-stained membrane are shown as loading controls. (b) BA sensitivity of an slt2 mutant strain compared to its WT BY4742 as determined by a spot assay.
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