Exposure to Glycolytic Carbon Sources Reveals a Novel Layer of Regulation for the MalT Regulon

Figure 4

Effect of carbon sources on malT ^con transcription. Semiquantitative RT-PCR of malT ^con in the ompR malT ^con double mutant (strain AJW3098) grown under nonpermissive conditions in the absence (lane 1–5) or presence of glucose (lane 6–10), maltose (lane 11–15), or sorbitol (lane 16–20). PCR amplification was carried out with a dilution series of the cDNA: undiluted (lane 1, 6, 11, and 16), 1 : 10 dilution (lane 2, 7, 12, 17), 1 : 25 dilution (lane 3, 8, 13, and 18), 1 : 125 dilution (lane 4, 9, 14, 19), and mock control (lane 5, 10, 15, and 20).