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International Journal of Microbiology
Volume 2011, Article ID 240191, 8 pages
http://dx.doi.org/10.1155/2011/240191
Research Article

Characterization of Halorubrum sfaxense sp. nov., a New Halophilic Archaeon Isolated from the Solar Saltern of Sfax in Tunisia

1Laboratoire de Chimie Bactérienne, Aix Marseille Université, UPR 9043-CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
2Laboratoire de Microbiologie, Faculté des Sciences de Sfax, BP802, Sfax 3000, Tunisia

Received 15 November 2010; Revised 2 March 2011; Accepted 31 March 2011

Academic Editor: Kaarina Sivonen

Copyright © 2011 Hana Trigui et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An extremely halophilic archaeon, strain ETD6, was isolated from a marine solar saltern in Sfax, Tunisia. Analysis of the 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus Halorubrum among the family Halobacteriaceae, with a close relationship to Hrr. xinjiangense (99.77% of identity). However, value for DNA-DNA hybridization between strain ETD6 and Hrr.xinjiangense were about 24.5%. The G+C content of the genomic DNA was 65.1 mol% (T(m)). Strain ETD6 grew in 15–35% (w/v) NaCl. The temperature and pH ranges for growth were 20–55°C and 6–9, respectively. Optimal growth occurred at 25% NaCl, 37°C, and pH 7.4. The results of the DNA hybridization against Hrr. xinjiangense and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain ETD6 from other Hrr. species. Therefore, strain ETD6 represents a novel species of the genus Halorubrum, for which the name Hrr. sfaxense sp. nov. is proposed. The Genbank EMBL-EBI accession number is GU724599.