International Journal of Microbiology / 2011 / Article / Tab 1 / Review Article
Defining Established and Emerging Microbial Risks in the Aquatic Environment: Current Knowledge, Implications, and Outlooks Table 1 Methods used to detect VBNC state in waterborne microorganisms.
Method(s) Employed Reporting author(s)* Failure of microbial growth in culture media [5 ] Use of redox probes to detect microbial respiratory chain activity [6 , 7 ] Incorporation of radio-labelled substrates in culture media [8 ] Resuscitation in embryo of egg yolk [4 ] Detection in immunodeficient mice [9 ] Addition of antioxidants to culture media [10 ] RNA-based genotypic approaches (16S/23S rRNA, mRNA) [11 ] cDNA microarrays [2 ] In situ hybridisation (FISH), microradiography, epi-fluorescence microscopy, flow cytometry[12 ] Rapid enzyme assays [13 ] Oligonucleotide probes and tagged green fluorescent protein [14 ] Microbial quorum sensing [15 ]
*This is a representative list of authors citing use of named methods for detection of VBNC state in waterborne organisms and therefore does not convey all published work in this area.