Research Article

Suitability of Commercial Transport Media for Biological Pathogens under Nonideal Conditions

Table 2

ECL immunodetection of B. anthracis, Y. pestis, and VEE. A four plus system was used to indicate the number of tests that were positive according to ECL immunodetection. Duplicate swabs were extracted at each time point and set up into duplicate detection assays. Thus there is a total of four luminescent readings to assess whether B. anthracis, Y. pestis and VEE displayed positive (+) or negative (−) detection. As in Table 1, in an effort to consolidate the large number of samples being extracted, VEE collection did not start until day 7 and persisted only until day 21. Table entries where there is discordance between bacterial and viral collection points have been grayed out.

Biothreat agentStorage temp.Storage mediaDay 1Day 7Day 14Day 21Day 28Day 60

Bacillus anthracis−70°CPBST++++++++++++++++++++++++
Amies++++++++++++++++++++++++
Stuart++++++++++++++++++++++++
Dry++++++++++++++++++++++++
4°CPBST++++
Amies++++
Stuart++++
Dry++++++++++++++++++++++++
25°CPBST++++
Amies
Stuart++++
Dry
45°CPBST
Amies
Stuart
Dry

Yersinia pestis−70°CPBST++++++++++++++++++++++++
Amies++++++++++++++++++++++++
Stuart++++++++++++++++++++++++
Dry++++++++++++++++++++++++
4°CPBST++++++++++++++++++++++++
Amies++++++++++++++++++++++++
Stuart++++++++++++++++++++++++
Dry++++++++++++++++++++++++
25°CPBST++++++++++++++++++++++++
Amies++++++++++++++++++++++++
Stuart++++++++++++++++++++++++
Dry++++++++++++++++++++++++
45°CPBST++++++++++++++++++++++++
Amies++++++++++++++++++++++++
Stuart++++++++++++++++++++++++
Dry++++++++++++++++++++++++

VEE−70°CPBST++++++++++++
UTM++++++++++++
M4RT++++++++++++
Dry++++++++++++
4°CPBST++++++++++++
UTM++++++++++++
M4RT++++++++++++
Dry++++++++++++
30°CPBST++++++++++++
UTM++++++++++++
M4RT++++++++++++
Dry++++++++++++