Research Article
Bacillus Strains Most Closely Related to Bacillus nealsonii Are Not Effectively Circumscribed within the Taxonomic Species Definition
Table 4
Comparison of differential phenotypic traits for strain CBD 118, eight closely related strains, and most closely related type strains. Strains: 1, Bacillus circulans ATCC ; 2, Bacillus nealsonii DSM ; 3, CBD 118; 4, OSS 25; 5, P307; 6, P308; 7, C4T1F3B3; 8, IAFILS6; 9, AD5A; 10, U4A; 11, ADP4II. aApproximate mean diameter of 5 well-isolated colonies grown 24 + 2 h, 30°C on tryptic soy agar plates. bLarger, more opaque zone of hydrolysis for these strains. Subtle, <50% increase in diameter of sporangium for preponderance of cells field−1; Overt, ≥50% increase in diameter of sporangium for ≥25% of cells field−1; +: positive; −: negative; Scant: optical density just above threshold for unaided detection; v: variable, indicating both + and − reactions for strain in ≥4 tests. Scant and v were differentiated from both + and − reactions in pairwise tabulation of differential traits.
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