Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions

Figure 1

A diagram showing the position and the approach used to obtain full-length rrs gene. The shaded box represents the downstream partial sequence determined in a previous study [13], while the open box represents the upstream partial sequence determined in this study. Each sequence-specific reverse primer was used to retrieve the upstream partial sequence and to quantify the abundance of each of the uncultured bacteria when paired with bacterial primers 27f and 5330f, respectively.