Research Article
Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions
Table 1
Primers* used to retrieve full-length rrs gene sequences of the uncultured bacteria.
| | Partial sequences (GenBank accession no.) | Sequences () | Annealing position (E. coli numbering) | Amplicon length (bp) |
| | Lq-H1-18 (AY816400) | GAC ACA CCT GAT CTC TCA GGT T | 1006–1028 | 1,021 | | Ad-H1-89 (AY816504) | CGA CTT TGC TTC CCT CTG TTT | 1246–1266 | 1,259 | | Ad-C2-43 (AY816609) | CTC CAG AGT GCC CAT CCG AA | 1135–1154 | 1,147 | | Ad-C1-74 (AY816616) | GAA GGG ACC GGT TAA GGT C | 1013–1031 | 1,024 | | Lq-H2-71 (AY816389) | TTC TCG GCC CCA AAT TCG | 1466–1483 | 1,476 | | Ad-H1-14 (AY816508) | GAT TTG CTT ACC CTC GCG GGT TT | 1260–1282 | 1,275 | | Ad-H1-53 (AY816423) | CGT ATC TCT ACG GCT TTA C | 1002–1020 | 1,013 | | Ad-H1-75 (AY816420) | CAC ACC TTG TAT CTC TAC AAG C | 1006–1027 | 1,020 | | Ad-H2-90 (AY816432) | CTT CGA CAG CTG CCT CCT TA | 1451–1470 | 1,463 | | Lq-C1-28 (AY816538) | CCA GGG CCA TTA CAC CCT GT | 1005–1025 | 1,018 | | Lq-C2-16 (AY816578) | GAC TTT GCT TCC CTT TGT TTT G | 1245–1265 | 1,258 | | Lq-C2-58 (AY816550) | AGC CTC CGA TAC ATC TCT GC | 1010–1029 | 1,022 |
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*Only the primers that produced a single sequence were listed.
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