Research Article
Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions
Table 2
Primers and probe used for real-time PCR quantification of recognized rumen bacterial species, genera, or uncultured bacteria.
| Primers | Sequences () | Target | Annealing temperature (°C) | Amplicon length (bp) | References |
| 27f | AGA GTT TGA TCM TGG CTC AG | Bacteria |
54 |
1,535 |
[19] | 1525r | AAG GAG GTG WTC CAR CC | 340f | TCC TAC GGG AGG CAG CAG T |
Bacteria |
60 |
467 |
[20] | 806r | GGA CTA CCA GGG TAT CTA ATC CTG TT | TaqMan probe | 6-FAM-5′-CGT ATT ACC GCG GCT |
70 | GCT GGC AC-3′-TAMRA | Bac303f | GAA GGT CCC CCA CAT TG | Bacteroides and Prevotella |
56 |
418 |
[21] | Bac708r | CAA TCG GAG TTC TTC GTG | ARC787F | ATT AGA TAC CCS BGT AGT CC | Archaea |
60 |
273 |
[22] | ARC1059R | GCC ATG CAC CWC CTC T | Ra1281f | CCC TAA AAG CAG TCT TAG TTC G | R. albus |
55 |
175 |
[23] | Ra1439r | CCT CCT TGC GGT TAG AAC A | Fs-f | GGT ATG GGA TGA GCT TGC | F. succinogenes |
63 |
446 |
[24] | Fs-r | GCC TGC CCC TGA ACT ATC | 530f | GTG CCA GCM GCC GCG G | Butyrivibrio |
65 |
371 |
[19] and this study | Buty-900r | TGC GGC ACY GAC TCC CTA TG | Sel-Mit-f | TGC TAA TAC CGA ATG TTG | S. ruminantium and M. multacida |
53 |
513 |
[24] | Sel-Mit-r | TCC TGC ACT CAA GAA AGA | Ram-f | CAA CCA GTC GCA TTC AGA | Ruminobacter amylophilus |
57 |
642 |
[24] | Ram-r | CAC TAC TCA TGG CAA CAT | Rf154f-K | TCT GGA AAC GGA TGG TA | R. flavefaciens |
55 |
295 |
[23] | Rf425r-K | CCT TTA AGA CAG GAG TTT ACA A |
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