International Journal of Microbiology / 2011 / Article / Tab 2

Research Article

Populations of Select Cultured and Uncultured Bacteria in the Rumen of Sheep and the Effect of Diets and Ruminal Fractions

Table 2

Primers and probe used for real-time PCR quantification of recognized rumen bacterial species, genera, or uncultured bacteria.

PrimersSequences ( )TargetAnnealing temperature (°C)Amplicon length (bp)References

27fAGA GTT TGA TCM TGG CTC AGBacteria 54 1,535 [19]
1525rAAG GAG GTG WTC CAR CC
340fTCC TAC GGG AGG CAG CAG T Bacteria 60 467 [20]
806rGGA CTA CCA GGG TAT CTA ATC CTG TT
TaqMan probe6-FAM-5′-CGT ATT ACC GCG GCT 70
GCT GGC AC-3′-TAMRA
Bac303fGAA GGT CCC CCA CAT TGBacteroides and Prevotella 56 418 [21]
Bac708rCAA TCG GAG TTC TTC GTG
ARC787FATT AGA TAC CCS BGT AGT CCArchaea 60 273 [22]
ARC1059RGCC ATG CAC CWC CTC T
Ra1281fCCC TAA AAG CAG TCT TAG TTC GR. albus 55 175 [23]
Ra1439rCCT CCT TGC GGT TAG AAC A
Fs-fGGT ATG GGA TGA GCT TGCF. succinogenes 63 446 [24]
Fs-rGCC TGC CCC TGA ACT ATC
530fGTG CCA GCM GCC GCG GButyrivibrio 65 371 [19] and this study
Buty-900rTGC GGC ACY GAC TCC CTA TG
Sel-Mit-fTGC TAA TAC CGA ATG TTGS. ruminantium and M. multacida 53 513 [24]
Sel-Mit-rTCC TGC ACT CAA GAA AGA
Ram-fCAA CCA GTC GCA TTC AGARuminobacter amylophilus 57 642 [24]
Ram-rCAC TAC TCA TGG CAA CAT
Rf154f-KTCT GGA AAC GGA TGG TAR. flavefaciens 55 295 [23]
Rf425r-KCCT TTA AGA CAG GAG TTT ACA A

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