Fluorescence microscopy to determine adherent and internalized Cryptococcus neoformans on brain microvascular endothelial cells. C. neoformans serotype A strain H99 cells were prestained with FITC (green) before 4 hr incubation with brain endothelial cells and then counterlabelled with Calcofluor White (blue) to label extracellular adherent yeasts. (a) Merged image to reveal both endothelial cells and associated yeast cells, (b) and (c) Fluorescence images revealing the FITC and Calcofluor stained yeast cells, respectively.
