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International Journal of Microbiology
Volume 2012, Article ID 683193, 8 pages
Research Article

Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation

1Department of Biotechnology Engineering, Tatyasaheb Kore Institute of Engineering & Technology, Warananagar Panhala, Kolhapur, Maharashtra, 416113, India
2Department of Microbiology, K.R.P. Kanya Mahavidyalaya, Islampur, Walwa, Sangli, Maharashtra, 415414, India

Received 8 July 2011; Revised 28 September 2011; Accepted 4 October 2011

Academic Editor: Gregory M. Cook

Copyright © 2012 Rajashri D. Kamble and Anandrao R. Jadhav. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A thermoalkalophilic new species of Bacillus, similar to Bacillus arseniciselenatis DSM 15340, produced extracellular xylanase under solid state fermentation when wheat bran is used as carbon source. The extracellular xylanase was isolated by ammonium sulfate (80%) precipitation and purified using ion exchange chromatography. The molecular weight of xylanase was ~29.8 ;kDa. The optimum temperature and pH for the enzyme activity were 50°C and pH 8.0. The enzyme was active on birchwood xylan and little active on p-nitrophenyl xylopyranoside but not on Avicel, CMC, cellobiose, and starch, showing its absolute substrate specificity. For birchwood xylan, the enzyme gave a Km 5.26 ;mg/mL and Vmax 277.7 ;μmol/min/mg, respectively. In addition, the xylanase was also capable of producing high-quality xylo-oligosaccharides, which indicated its application potential not only in pulp biobleaching processes but also in the nutraceutical industry.