Research Article
Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation
Table 1
Morphological, physiological, and biochemical characteristics of the isolate.
| Tests | Results | Tests | Results |
| Colony morphology | Growth on NaCl (%) | Shape | Circular | 2.0 | − | Margin | Regular | 4.0 | − | Elevation | Raised | 6.0 | + | Consistency | Moist | 8.0 | + | Color | Pale yellow | 10.0 | − | Opacity | Opaque | Anaerobic Growth | Facultative | Gram nature | Gram Positive | Utilization of carbohydrates | Shape of the cell | Long rods | Xylose | + | Motility | Sluggish Motile | Lactose | + | Endospore position | Central | Mannitol | − | Growth at temperatures | Arabinose | − | 10°C | − | Sucrose | − | 25°C | − | Glucose | − | 30°C | + | Fructose | + | 37°C | + | Melibiose | − | 45°C | +++ | Starch hydrolysis | + | 60°C | + | Gelatin hydrolysis | + | 70°C | – | Urea hydrolysis | − | Growth at pH | Esculin hydrolysis | − | 5.0 | − | Casein hydrolysis | − | 6.0 | − | Tween 20 hydrolysis | + | 7.0 | + | Catalase test | + | 8.0 | ++ | Oxidase test | + | 10.0 | − | Nitrate reduction | + | | | H2S production | − |
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+: Positive; −: Negative.
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