Temperature Depended Role of Shigella flexneri Invasion Plasmid on the Interaction with Acanthamoeba castellanii
Figure 4
Electron microscopy at 30°C, black arrows point out necrosis and white apoptosis. A. castellanii trophozoite cultivated alone (a), A. castellanii trophozoite cocultivated with wild type S. flexneri showing necrosis (b), A. castellanii cyst cocultivated with plasmid-cured S. flexneri (c), and A. castellanii trophozoite cocultivated with plasmid-cured S. flexneri showing apoptosis (d).