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Primer name | Nucleotide sequence |
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Mutans DHPSph |
5′-GAT CGA TCG CAT GCA CAT CAT AAC TAG GGA GCA AGC-3′ |
mutansDHPSBam | 5′-GAT GGA TCG GAT CCA AAA TAATCT TAT CCA TAA CAC CCT CA-3′ |
dhpssfwph | 5′-AAC CTA CTG CAT GCA TAA GAA TCA G-3′ |
dhpssreveco | 5′-ATT GTA GGA ATT CTT CTA GAA AGA TCC-3′ |
downeifolpfw | 5′-GCA TGC CAA AGA CAG GAA TTG CTG AC-3′ |
Downeifolprevps | 5′-CTG CAG CCA CAA AAA TTT GCC CCA GAC-3′ |
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| Primers for changing specific amino acids in isolate 797 |
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DHPS46AVfw | 5′-TGA AGC CAT GTT AGT AGC AGG AGC GGC TA-3′ |
DHPS46AVrev | 5′-TAG CCG CTC CTG CTA CTA ACA TGG CTT CA-3′ |
DHPS80aEKfw | 5′-TCG TTC CAA TTG TTA AAG CTA TTA GCG AA-3′ |
DHPS80aEKrev | 5′-TTC GCT AAT AGC TTT AAC AAT TGG AAC GA-3′ |
DHPS122QHfw | 5′-CTT TAT GAT GGG CAC ATG TTT CAA TTA GC-3′ |
DHPS122QHrev | 5′-GCT AAT TGA AAC ATG TGC CCA TCA TAA AG-3′ |
DHPS146SGfw | 5′-GTG AAG AAG TTT ATG GCA ATG TAA CAG AA-3′ |
DHPS146SGrev | 5′-TTC TGT TAC ATT GCC ATA AAC TTC TTC AC-3′ |
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| Primers for changing specific amino acids in isolate 8 |
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V37Afw | 5′-AAC CAA TCG ATC AGG CTC TAA AAC AGG TTG A-3′ |
V37Arev | 5′-TCA ACC TGT TTT AGA GCC TGA TCG ATT GTT T-3′ |
D172Nfw | 5′-GGA GTT AAA AAA GAA AAT ATT TGG CTT GAT C-3 |
D172Nrev | 5′-GAT CAA GCC AAA TAT TTT CTT TTT TAA CTC C-3′ |
Q193Rfw | 5′-ACA TGG AAC TTC TAC GAG GCT TAG CGG AGG T-3 |
Q193Rrev | 5′-ACC TCC GCT AAG CCT CGT AGA AGT TCC ATG T-3′ |
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