Table 4: Box-Behnken design matrix along with the experimental and predicted responses (antibiotic activity) of Streptomyces sp. JAJ06.

RunsVariables/coded valuesAntibiotic activity (U/mL) SEM
StarchKBrCaCO3ExperimentalPredicted

10 108.32
2+0 136.25
3+0 123.75
4++0 149.97
50 127.29
6+0 149.36
70+ 110.64
8+0+ 142.71
90 124.39
100+ 137.26
110+ 111.04
120++ 127.31
13000 165.34
14000 165.34
15000 165.34
16000 165.34
17000 165.34