Bacteriophage-Derived Peptidase Eliminates and Prevents Staphylococcal Biofilms
Figure 3
biofilm prevention assay: staining. Concentrations of enzyme ranging from 0–125 μg mL−1 were incubated with S. aureus DPC5246 in TSBg at 37°C for 24 h. The wells were stained with crystal violet (1%) and subsequently the stain was solubilised with acetic acid. Optical density readings at 590 nm (OD590) of all wells were recorded in a microtitre plate reader and displayed on the bar chart. Assays were carried out in triplicate and OD590 data was expressed as the mean ± SE.
