Research Article
Design and Optimization of a Process for Sugarcane Molasses Fermentation by Saccharomyces cerevisiae Using Response Surface Methodology
Table 2
Experimental design matrix with experimental and predicted values of bioethanol yield.
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Run number | Factors/Levels | | | | Incubation period h | Initial pH | Incubation temperature °C | Molasses conc wt.% | Bioethanol yield | | | | | g/L | | Coded value | Actual value | Coded value | Actual value | Coded value | Actual value | Coded value | Actual value | Experimental data | Predicted data |
| | 1 | +1 | 72.00 | −1 | 5.00 | +1 | 40.00 | +1 | 25.00 | 88.86 | 90.14 | | 2 | 0 | 48.00 | 0 | 6.00 | +1 | 40.00 | 0 | 20.00 | 243.32 | 224.85 | | 3 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 178.89 | 177.44 | | 4 | +1 | 72.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 192.03 | 183.22 | | 5 | −1 | 24.00 | +1 | 7.00 | −1 | 20.00 | +1 | 25.00 | 220.45 | 207.73 | | 6 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | +1 | 25.00 | 110.22 | 108.58 | | 7 | 0 | 48.00 | 0 | 6.00 | −1 | 20.00 | 0 | 20.00 | 200.00 | 218.18 | | 8 | +1 | 72.00 | −1 | 5.00 | +1 | 40.00 | −1 | 15.00 | 230.93 | 232.68 | | 9 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 177.00 | 177.44 | | 10 | +1 | 72.00 | −1 | 5.00 | −1 | 20.00 | −1 | 15.00 | 74.39 | 81.02 | | 11 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | −1 | 15.00 | 112.00 | 113.36 | | 12 | 0 | 48.00 | +1 | 7.00 | 0 | 30.00 | 0 | 20.00 | 86.00 | 84.45 | | 13 | −1 | 24.00 | +1 | 7.00 | −1 | 20.00 | −1 | 15.00 | 65.00 | 74.75 | | 14 | −1 | 24.00 | −1 | 5.00 | −1 | 20.00 | −1 | 15.00 | 36.73 | 14.50 | | 15 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 176.00 | 177.44 | | 16 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 179.00 | 177.44 | | 17 | +1 | 72.00 | +1 | 7.00 | −1 | 20.00 | −1 | 15.00 | 12.00 | 8.01 | | 18 | +1 | 72.00 | −1 | 5.00 | −1 | 20.00 | +1 | 25.00 | 10.00 | 4.04 | | 19 | −1 | 24.00 | −1 | 5.00 | +1 | 40.00 | +1 | 25.00 | 5.00 | 1.98 | | 20 | +1 | 72.00 | +1 | 7.00 | +1 | 40.00 | −1 | 15.00 | 50.00 | 49.27 | | 21 | −1 | 24.00 | −1 | 5.00 | −1 | 20.00 | +1 | 25.00 | 14.18 | 25.95 | | 22 | −1 | 24.00 | +1 | 7.00 | +1 | 40.00 | −1 | 15.00 | 7.00 | 1.99 | | 23 | +1 | 72.00 | +1 | 7.00 | +1 | 40.00 | +1 | 25.00 | 17.00 | 28.27 | | 24 | 0 | 48.00 | −1 | 5.00 | 0 | 30.00 | 0 | 20.00 | 84.00 | 85.26 | | 25 | −1 | 24.00 | +1 | 7.00 | +1 | 40.00 | +1 | 25.00 | 65.00 | 69.41 | | 26 | −1 | 24.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 162.00 | 170.53 | | 27 | +1 | 72.00 | +1 | 7.00 | −1 | 20.00 | +1 | 25.00 | 54.00 | 52.56 | | 28 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 174.00 | 177.44 | | 29 | −1 | 24.00 | −1 | 5.00 | +1 | 40.00 | −1 | 15.00 | 39.66 | 52.13 | | 30 | 0 | 48.00 | 0 | 6.00 | 0 | 30.00 | 0 | 20.00 | 178.90 | 177.44 |
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