Research Article

Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity

Figure 2

Screening A. oryzae culture supernatants for antibacterial activity. Fungal culture in 96 well plates allows visible confirmation of fungal growth. As seen in (a), wells with fungal growth are dark, while wells with no fungal growth contain only culture media and are comparatively clear. Examination of U-bottom 96 well plate bacterial cultures subjected to fungal culture supernatants allows visual evaluation of reduction in MRSA bacterial growth (b). (c) a magnified area (boxed) of image in (b) clearly showing a single well with extremely reduced bacterial growth indicating antibacterial activity of fungal culture supernatant.
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(a)
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(b)
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(c)