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International Journal of Microbiology
Volume 2014, Article ID 276941, 6 pages
http://dx.doi.org/10.1155/2014/276941
Research Article

Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran

1Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah 67148-69914, Iran
2Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, P.O. Box 678, Amol, Iran
3Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah 67148-69914, Iran

Received 8 January 2014; Revised 20 March 2014; Accepted 21 March 2014; Published 15 April 2014

Academic Editor: Giuseppe Comi

Copyright © 2014 Parviz Mohajeri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objective. Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Molecular fingerprinting of UTI isolates such as pulsed-Field Gel Electrophoresis using for Clonal distribution and determine of predominant type. The aim of the study was to determine genotyping of ESBL producing UPECs. Material and Methods. 200 UPEC isolates from outpatients with UTI were obtained. Antimicrobial susceptibility and interpretation were performed by disk diffusion. Virulence factors for UPECs were screened by using PCR. UPECs were analyzed by Pulsed-Field Gel Electrophoresis and images analyzed by Phoretix1DPro software. Results. A total of 200 isolates of UPECs, 24.5% ( ) of isolates, were positive for ESBL production. Resistance ranged from 0% for amikacin and imipenem to over 93.9% for carbenicillin and ampicillin. Frequencies of haemagglutination, haemolysin, and hydrophobicity were 51%, 18.3%, and 14.28%, respectively. A total of 10 different genotypes were obtained, which include nine common clones and one single clone. Conclusion. We confirmed the prevalence of virulence phenotyping especially Haemagglutination among UPEC strains and that it can also contribute to virulence in these strains. Large diversity in genotypes was observed in the isolates that could be indicative of different sources of infection in community acquired.