Prevalence of Clinical Periodontitis and Putative Periodontal Pathogens among South Indian Pregnant Women

Table 1

Species specific primers used in our study.

Name of bacteria	Forward primer (5′-3′)	Reverse primer (5′-3′)	𝑇𝑎^∗∗  (°C)	Amplicon size (bp)
Pg	TGTAGATGACTGATGGTGAAAACC	ACGTCATCCCCACCTTCCTC	60	197
Tf	GCGTATGTAACCTGCCCGCA	TGCTTCAGTGTCAGTTATACCT	60	641
Pi	TTTGTTGGGGAGTAAAGCGGG	TCAACATCTCTGTATCCTGCGT	55	575
Pn	ATGAAACAAAGGTTTTCCGGTAAG	CCCACGTCTCTGTGGGCTGCGA	55	804
Cr	TTTCGGAGCGTAAACTCCTTTTC	TTTCTGCAAGCAGACACTCTT	60	598
Ec	CTAATACCGCATACGTCCTAAG	CTACTAAGCAATCAAGTTGCCC	60	688
Aa	AGAGTTTGATCCTGGCTCAG	CACTTAAAGGTCCGCCTACGTGCC	60*	593
Co	AGAGTTTGATCCTGGCTCAG	GATGCCGTCCCTATATACGGGG	55	185
Cs	AGAGTTTGATCCTGGCTCAG	GATGCCGCTCCTATATACCATTAGG	55*	185
Td	AAGGCGGTAGAGCCGCTCA	AGCCGCTGTCGAAAAGCCCA	55	311

∗Confirmation was done using a separate run of PCR to differentiate from other bacteria in the same batch of PCR producing similar size of amplicons.
^∗∗𝑇_𝑎: Annealing temperature.