Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae

Table 1

Sequences of the primers used to detect 𝛽-lactamases genes.

PCR target	Primer name	Primer sequence	Annealing temperatures on °C	Amplicon sizes
CTX-M-1 group	CTX-1F	5′ATGGTTAAAAAATCACTGCGTC3′	60	864 pb
	CTX-1R	5′TTGGTGACGATTTTAGCCGC3′	60
CTX-M-2 group	CTX-2F	5′ATGATGACTCAGAGCATTCG3′	58	866 pb
	CTX-2R	5′TGGGTTACGATTTTCGCCGC3′	62
CTX-M-8 group	CTX-8F	5′ACTTCAGCCACACGGATTCA3′	60	877 pb
	CTX-8R	5′CGAGTACGTCACGACGACTT3′	62
CTX-M-9 group	CTX-9F	5′ATGGTGACAAAGAGAGTGCAA3′	60	876 pb
	CTX-9R	5′TCACAGCCCTTCGGCGATGATTCTCGC3′	86
SHV	SHV-1F	5′ATGCGTTATATTCGCCTGTGTATT3′	66	868 pb
	SHV-1R	5′-TTAGCGTTGCCAGTGCTCGATCAG-3′	74
TEM	TEM-1F	5′ATAAAATTCTTGAAGACGAAA3′	52	1080 pb
	TEM-1R	5′GACAGTTACCAATGCTTAATC3′	58