Research Article
Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae
Table 1
Sequences of the primers used to detect
-lactamases genes.
| PCR target | Primer name | Primer sequence | Annealing temperatures on °C | Amplicon sizes |
| CTX-M-1 group | CTX-1F | 5′ATGGTTAAAAAATCACTGCGTC3′ | 60 | 864 pb | CTX-1R | 5′TTGGTGACGATTTTAGCCGC3′ | 60 |
| CTX-M-2 group | CTX-2F | 5′ATGATGACTCAGAGCATTCG3′ | 58 | 866 pb | CTX-2R | 5′TGGGTTACGATTTTCGCCGC3′ | 62 |
| CTX-M-8 group | CTX-8F | 5′ACTTCAGCCACACGGATTCA3′ | 60 | 877 pb | CTX-8R | 5′CGAGTACGTCACGACGACTT3′ | 62 |
| CTX-M-9 group | CTX-9F | 5′ATGGTGACAAAGAGAGTGCAA3′ | 60 | 876 pb | CTX-9R | 5′TCACAGCCCTTCGGCGATGATTCTCGC3′ | 86 |
| SHV | SHV-1F | 5′ATGCGTTATATTCGCCTGTGTATT3′ | 66 | 868 pb | SHV-1R | 5′-TTAGCGTTGCCAGTGCTCGATCAG-3′ | 74 |
| TEM | TEM-1F | 5′ATAAAATTCTTGAAGACGAAA3′ | 52 | 1080 pb | TEM-1R | 5′GACAGTTACCAATGCTTAATC3′ | 58 |
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