Study of a Natural Mutant SHV-Type β-Lactamase, SHV-104, from Klebsiella pneumoniae

Table 2

MICs of various antimicrobial agents obtained for the clinical isolate K.  pneumoniae ML2011, transformants, transconjugants, and the E. coli recipients.

Antibiotics	K.  pneumoniae	E. coli
	pML2011	HB 101	HB 101 × pML2011	DH5𝛼	DH5𝛼/pML2011	BL21/pET26b	BL21/pET26b 𝑏𝑙𝑎SHV-104
Ampicillin	>512	4	>512	8	>512	8	>512
Amoxicillin	>512	4	>512	8	>512	8	>512
Ticarcillin	>512	2	>512	2	>512	2	>512
Cephaloridine	>512	1	>512	0.13	>512	ND	ND
Cefoxitin	64	2	8	4	8	2	2
Cefotaxime	>512	1	512	0.13	512	2	8
Ceftazidime	256	1	64	0.13	64	2	2
Ceftriaxone	>512	1	512	0.13	512	2	2
Cefpirome	>512	2	>512	0.13	>512	ND	ND
Aztreonam	256	1	256	0.13	256	ND	ND
Imipenem	2	0.25	0.38	0.06	0.38	ND	ND
Chloramphenicol	>256	2	2	2	2	4	4
Nalidixic acid	>512	1	1	0.06	1	1	1
Ciprofloxacin	256	1	1	0.06	1	ND	ND
Tetracycline	>512	2	2	0.25	2	ND	ND

ND: not determinable.