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International Journal of Microbiology
Volume 2014, Article ID 615965, 7 pages
http://dx.doi.org/10.1155/2014/615965
Research Article

High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus

Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still University of Health Sciences, 800 West Jefferson Street, Kirksville, MO 63501, USA

Received 29 July 2013; Revised 16 September 2013; Accepted 24 October 2013; Published 19 January 2014

Academic Editor: Barbara H. Iglewski

Copyright © 2014 Vineet K. Singh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Staphylococcus aureus is a major human and animal pathogen. Autolysins regulate the growth, turnover, cell lysis, biofilm formation, and the pathogenicity of S. aureus. Atl is the major autolysin in S. aureus. The biochemical and structural studies of staphylococcal Atl have been limited due to difficulty in cloning, high level overexpression, and purification of this protein. This study describes successful cloning, high level over-expression, and purification of two forms of fully functional Atl proteins. These pure proteins can be used to study the functional and structural properties of this important protein.