High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus

Figure 1

Diagrammatic representation of full-length staphylococcal atl and various shortened atl gene fragments that were cloned for overexpression in E. coli. The full length Atl (~137 kDa) possesses a signal peptide, a propeptide, and two catalytically active amidase and glucosaminidase domains.