High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus

Figure 4

Coomassie stained gels showing purity of recombinant His-tagged Atl. Recombinant Atl and Atl-1 proteins were overproduced in E. coli and purified as described in the Materials and Methods section. Lane M: standard protein markers; lanes 1 and 2: protein extract from E. coli BL21 cells transformed with plasmids pRSETA-atl and grown without and with IPTG, respectively; lane 3: purified His-tagged Atl; lanes 4 and 5: protein extract from E. coli BL21 cells transformed with plasmids pRSETA-atl1 and grown without and with IPTG, respectively; lane 6: purified His-tagged Atl-1.