High Level Expression and Purification of Atl, the Major Autolytic Protein of Staphylococcus aureus

Figure 5

Zymographic detection of the autolytic activity in purified recombinant His-tagged Atl. Lane 1: total protein extract from S. aureus cells. Lanes 2 and 4: purified His-tagged Atl and Atl-1, respectively; lanes 3 and 5: protein extract from E. coli BL21 cells transformed with plasmids pRSETA-atl or pRSETA-atl1 and grown with IPTG, respectively.