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International Journal of Microbiology
Volume 2014, Article ID 979584, 8 pages
http://dx.doi.org/10.1155/2014/979584
Research Article

Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains

1Programa de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil
2Departamento de Medicina Veterinária Preventiva, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil
3Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil
4Departamento de Morfologia, Fisiologia e Patologia Básica, FORP, Avenida do Café S/N, Campus USP, 14040-904 Ribeirão Preto, SP, Brazil

Received 23 July 2013; Revised 2 December 2013; Accepted 23 January 2014; Published 9 March 2014

Academic Editor: José M. Pérez-Donoso

Copyright © 2014 Juliana M. A. Agostinho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.