Influence of Vinasse Application in the Structure and Composition of the Bacterial Community of the Soil under Sugarcane Cultivation

Table 2

Bacterial phylotypes distribution in clone libraries of 16S rRNA gene as affiliation by Classifier algorithm available on RDP II using cutting 80% confidence.

Phylogenetic groups	Clone library (%)
	SPV	SPS	SMC	SFS	Total
Proteobacteria	30.3	62.9	40.0	59.6	48.2
Alphaproteobacteria	22.1	56.8	19.3	55.0	38.3
Betaproteobacteria	5.0	1.8	0.4	0.4	2.0
Gammaproteobacteria	—	0.7	6.4	—	1.8
Deltaproteobacteria	0.4	—	—	0.4	0.2
Unclassified Proteobacteria	2.9	3.2	13.9	3.6	6.0
Acidobacteria	28.2	8.9	13.2	6.1	14.1
Actinobacteria	6.4	20.7	13.9	14.6	13.9
Firmicutes	2.9	0.7	7.1	—	2.7
Gemmatimonadetes	1.4	0.7	0.3	2.5	1.3
Others	11.0	1.0	—	—	2.9
Unclassified bacteria	20.0	5.0	25.0	17.1	17

Abbreviations of the four biomes are shown in Table 1.
Others: Planctomycetes, Nitrospirae, Bacteroidetes, Chloroflexi, Armatimonadetes, Candidatus phyla Saccharibacteria, and Candidatus phyla division WPS-1.
Normalization factor is 280/total, where total is sum of the number of sequences not chimeras. The normalization factor of each environment was multiplied by each taxon observed at the level of phylum and converted to percentage.