Penicillin G-Induced Chlamydial Stress Response in a Porcine Strain of Chlamydia pecorum

Table 1

Chlamydia pecorum recovers infectious elementary body production (inclusion-forming units/mL) upon discontinuation of penicillin G exposure.

Hours	Experimental group	Experiment  1		Experiment  2
24	Mock
	PenG 0 hpi	3.25  ×    ±  1.77  ×	0.0003^#	1.00  ×   ±    ×	0.0002^#
	PenG 0 hpi recovery		0.0016^##		0.0825^##
	PenG 14 hpi	1.75  ×    ±  3.54  ×	0.0003^#	3.00  ×    ±    ×	0.0002^#
	PenG 14 hpi recovery		0.0037^##		0.4196^##
48	Mock
	PenG 0 hpi	5.50  ×    ±  2.83  ×	0.0026^#	3.00  ×    ±  7.07  ×	0.0019^#
	PenG 0 hpi recovery		0.1345^##		0.0015^##
	PenG 14 hpi	×	0.1314^#	6.75  ×    ±    ×	0.0249^#
	PenG 14 hpi recovery		<0.0001^##		0.2091^##

C. pecorum 1710S-infected HeLa cells were exposed to diluent only (mock) or to penicillin G at 0 hours postinfection (hpi) or 14 hpi. At 35 hpi, medium was changed and exposure was continued or discontinued (recovery) for 24 or 48 hours. Inclusion counts generated from biological duplicates were used to calculate mean inclusion-forming units/mL. Results from 2 independent experiments evaluating 24 h of recovery or 48 h of recovery are shown. values for groups compared to corresponding mock (#) or corresponding continued exposure groups (##) are shown; = significant.