Novel Microdilution Method to Assess Double and Triple Antibiotic Combination Therapy In Vitro

Figure 1

Configuration of the chemostat-like model to evaluate double and triple antibiotic combinations by time-kill assay in vitro. The bottles were connected to two IV infusion pumps via IV infusion sets. The first pump delivers fresh medium to the bottles at 10 mL/h to provide nutrients and continuously dilute the antibiotics. A second pump was connected to the bottle through disposable syringe bacterial filter to withdraw the medium without the bacteria at 10 mL/h flow rate.