Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant

Table 2

Comparison of the gene transcription in wild type C. perfringens ATCC 13124 and gatifloxacin resistant mutant by qRT-.

Locus	Gene	Primer	Fold (mutant/wild)
CPF_0541 upstream	treB upstream	ACAGAGTACATAGGCGGAAAAGA	−333.89	−828.5	−330
		TTTCAACATCTGCTTTGCTTG
CPF_0541	treB	TTGGGACTTTGGATTTGCTC	−93.81	−227.5	−22.28
		GCCTGCCACTATAGCTCCTG
CPF_0542	treC	GAGCTTTAAAGGGCGAAGAAA	−45.1	−44.50	−16.80
		CCCAGTTTAAATCAGCCTGTG
CPF_0543	treR	GCCTTCAGAATCACAACTTATGG	−1.69	−1.79	−1.31
		TTTCGCACTCTTTTCCTAAGC
CPF_0539	RNA polymerase sigma-70 factor	TGTTGGATGAAAGTACACCGA	2.29	3.63	2.99
		AGCCCTCCTTTAAAACCTCA
CPF_0069	Transcription antiterminator	ATTCGGCAAGAACAACAGGA	−90.74	−711.9	−116.6
		GCAACCTTAAAGGATTCTGGA
CPF_0042	plc	TGACACAGGGGAATCACAAA	−9.58	−14.20
		CGCTATCAACGGCAGTAACA
CPF_0623	revR	AGTCCTAATAGTAGATGATGAGGA	−1.92	−1.73	−1.51
		CTTATAAGCTTTAGAGCTTCAGT
CPF_0007	gyrA	TGCCAGAATAGTTGGGGAAG	1.08	1.27
		TACCATGTCCGTCAACAAGC
CPF_1204	vrr	CAAAAAGGATTTTAACAAGTGCAA	−596.80	−133.1
		TTGATATTAAAGCAAGTATGGGACT
CPF_1956	topA	ACCTTGCAACTGACCCTGAT	−2.38	−2.42	−2.07
		CAGCACTCAATCCCCATTTT
CPF_1760	topB	AACTTGGGCTTTAGGCCATT	−1.61
		GCTACGAGCTCCCCTTCTCT
CPF_1785	PTS sucrose-specific IIBC component	GCGGCCATATTTGGAGTAAA	−92.15
		CCAGTTCCAGCCAATTTCAT

treB and treC are trehalose-specific genes. The transcription of several genes was considerably decreased in the mutant in comparison with the wild type ATCC 13124; the data presented represent those obtained in different experiments. The expression of topoisomerase genes (gyr and top) and response regulator gene (revR) was not markedly changed. Pfo, which was also downregulated, was reported previously [28].