Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil
Table 1
Primer sequences used for the identification of Escherichia coli virulence.
Serotypes
Oligonucleotides
Gene
Sequences (5′-3′)
Fragment size (bp)
EPEC
EAE-a
eaeA
ATG CTT AGT GCT GGT TTA GG
248
EAE-b
GCC TTC ATC ATT TCG CTT TC
EHEC
JMS1-F
stx1
GTC ACA GTA ACA AAC CGT AAC A
95
JMS1-R
TCG TTG ACT ACT TCT TAT CTG GA
ETEC
LT-1
lt
AGC AGG TTT CCC ACC GGA TCA CCA
132
LT1-2
GTG CTC AGA TTC TGG GTC TC
Sta-F
st
GCT AAT GTT GGC AAT TTT TAT TTC TGT A
190
Sta-R
AGG ATT ACA ACA AAG TTC ACA GCA GTA A
EAEC
Aggrks-1
aggR
GTA TAC ACA AAA GAA GGA AGC
254
Aggrks-2
ACA GAA TCG TCA GCA TCA GC
East1s
astA
GAG TGA CGG CTT TGT AGT CC
106
East1sa
GCC ATC AAC ACA GTA TAT CC
EIEC
VirA-F
virA
CTG CAT TCT GGC AAT CTC TTC ACA
215
VirA-R
TGA TGA GCT AAC TTC GTA AGC CCT CC
The pathotypes and virulence genes for the E. coli detected in this study are EPEC: enteropathogenic E. coli, EHEC: enterohemorrhagic E. coli, ETEC: enterotoxigenic E. coli, EAEC: enteroaggregative E. coli, and EIEC: enteroinvasive E. coli (adapted from Bisi Johnson et al. 2011 [27]).