Microbiological Quality Assessment by PCR and Its Antibiotic Susceptibility in Mangrove Crabs (Ucides cordatus) from Guanabara Bay, Rio de Janeiro, Brazil

Table 1

Primer sequences used for the identification of Escherichia coli virulence.

Serotypes	Oligonucleotides	Gene	Sequences (5′-3′)	Fragment size (bp)
EPEC	EAE-a	eaeA	ATG CTT AGT GCT GGT TTA GG	248
	EAE-b		GCC TTC ATC ATT TCG CTT TC
EHEC	JMS1-F	stx1	GTC ACA GTA ACA AAC CGT AAC A	95
	JMS1-R		TCG TTG ACT ACT TCT TAT CTG GA
ETEC	LT-1	lt	AGC AGG TTT CCC ACC GGA TCA CCA	132
	LT1-2		GTG CTC AGA TTC TGG GTC TC
	Sta-F	st	GCT AAT GTT GGC AAT TTT TAT TTC TGT A	190
	Sta-R		AGG ATT ACA ACA AAG TTC ACA GCA GTA A
EAEC	Aggrks-1	aggR	GTA TAC ACA AAA GAA GGA AGC	254
	Aggrks-2		ACA GAA TCG TCA GCA TCA GC
	East1s	astA	GAG TGA CGG CTT TGT AGT CC	106
	East1sa		GCC ATC AAC ACA GTA TAT CC
EIEC	VirA-F	virA	CTG CAT TCT GGC AAT CTC TTC ACA	215
	VirA-R		TGA TGA GCT AAC TTC GTA AGC CCT CC

The pathotypes and virulence genes for the E. coli detected in this study are EPEC: enteropathogenic E. coli, EHEC: enterohemorrhagic E. coli, ETEC: enterotoxigenic E. coli, EAEC: enteroaggregative E. coli, and EIEC: enteroinvasive E. coli (adapted from Bisi Johnson et al. 2011 [27]).