Gene Expression and Molecular Characterization of a Xylanase from Chicken Cecum Metagenome

Figure 4

Purification steps of and zymogram analysis. Proteins were separated with 12% gel SDS-PAGE. Lane (Zym) zymogram, lane (C): total soluble cell lysate from E. coli host expressing , lane (Co): Trx-His- fusion protein purified by TALON cobalt resin, lane (E): purified after digestion with enterokinase, and lane (M): protein molecular weight markers.