International Journal of Microbiology / 2017 / Article / Tab 1 / Research Article
Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, BrazilTable 1 Primers used for screening of resistance determinants in Staphylococcus saprophyticus .
Antimicrobial Gene Sequence 5′-3′ Size (bp) Ref. Gentamicin aac(6 ′)-Ie+aph(2 ′′) CCAAGAGCAATAAGGGCATACC 347 [19 ] CACACTATCATAACCATCACCG ant(4 ′)-Ia CTGCTAAATCGGTAGAAGC 172 CAGACCAATCAACATGGCACC aph(3 ′)-IIIa CTGATCGAAAAATACCGCTGC 268 TCATACTCTTCCGAGCAAAGG Erythromycin erm (A)TATCTTATCGTTGAGAAGGGATT 533 [20 ] CTACACTTGGCTTAGGATGAAA erm (B)CTATCTGATTGTTGAAGAAGGATT 359 [21 ] GTTTACTCTTGGTTTAGGATGAAA erm (C)CTTGTTGATCACGATAATTTCC 190 [22 ] ATCTTTTAGCAAACCCGTATTC msr (A)GGCACAATAAGAGTGTTTAAAGG 940 [21 ] AAGTTATATCATGAATAGATTGTCCTGTT msr (B)TATGATATCCATAATAATTATCCAATC 595 AAGTTATATCATGAATAGATTGTCCTGTT lin (A)GGTGGCTGGGGGGTAGATGTATTAACTGG 323 GCTTCTTTTGAAATACATGGTATTTTTCGATC mph (C)GAGACTACCAAGAAGACCTGACG 722 [23 ] CATACGCCGATTCTCCTGAT mef (A)AGTATCATTAATCACTAGTGC 348 [24 ] TTCTTCTGGTACAAAAGTGG Oxacillin mecA GTGAAGATATACCAAGTGATT 147 [25 ] ATGCGCTATAGATTGAAAGGAT Penicilli blaZ ATGTAATTCAAACAGTTCACATGCC 701 [26 ] ATAGGTTCAGATTGGCCCTTAGG pbp1A This study Fragment 1 GGCGAACATTCCACAGTGTTGACT 627 GGGTGTCAGTAAGGCGTTCAAACCA Fragment 2 TCTCACCGTCACCAATAACGATTGG 680 TACAGGCGGATCGCTTGGG Fragment 3 TCAGCTACTTGTGCGGTACCTGT 758 TTTGCAGTAGTGATGGACGCACA Fragment 4 GCCGCCTGTAGCTTCACCGT 650 TGAAAGGTGCTATGGGCGTAGAGAA Fragment 5 TGTGCGTCCATCACTACTGCAAA 788 GGGGCAGTCCTACTCATCCTTGGA Fragment 6 GCCGACATATCAACAACGGTAGCCA 683 AGACCAAAACCGTAAAACGCAAGGT pbp2A This study Fragment 1 ACGTGCTGCACTTTGTTGGTTACT 775 TGCCGGAAACGATGCACCAA Fragment 2 TTGGTGCATCGTTTCCGGCA 763 GCGGAATCTGCTTATCTTGCTGGT Fragment 3 CGCGATCTTCAGATGAACGTTGGA 869 GCGAGGACCGCGTATGACGG pbp3A This study Fragment 1 GACGATTATGACGGCCTTTT 702 TGGATCAAGTGCAGAACCAG Fragment 2 CCTGACAGTCAACGAAGCTG 769 AAGATCATCGCCACGTGAAC Fragment 3 GCATTTTCGGTGACGTTTCT 714 CGCTCGTAATATCGGTTGGT Fragment 4 TGATTGATGAGCCGCTTAAA 744 ATGGCTCAGGTACTGGTTGG Fragment 5 GGCATTTAACGAAAAAGATGGA 410 TACGTTTACGCGCATGCTAA pbp4A This study Fragment 1 AATCCAGCGACAAACATCCCATTCA 835 AAGCGCAGCAGCATTACTAGAGTT Fragment 2 GGCGCCATTAATGTTTCGCAAACA 718 CGCCGGCGCCCATGATAACT Fragment 3 GGTGCCGATATGAGCCTAGAAGGT 859 TGGGCCATGATTGGGAAGGCG Trimethoprim dfrD CCCTGCTATTAAAGCACC 606 [27 ] CATGACCAGATAACTC dfrG TGCTGCGATGGATAAGAA 405 [26 ] TGGGCAAATACCTCATTCC dfrK CAAGAGATAAGGGGTTCAGC 229 ACAGATACTTCGTTCCACTC dfrS1 (dfrA )CACTTGTAATGGCACGGAAA 270 CGAATGTGTATGGTGGAAAG dfr b AATGGACATCGGTTGGGTTGCCT 484 This study CGCACCGGATTCAAATGTCTCGC Norfloxacin gyrA CGAGTGAGATGCGCGAGTCATTCTT 731 This study ACGTTGACGACCGCCACCAC parC ACGTTCGTGATGGGCTCAAACCT 797 ACGTAATCCAGTACGGTCTGTCTCA
Ref.: reference. pbp 1-4 gene fragments and allow for the respective whole gene sequence amplification; dihydrofolate reductase .
