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International Journal of Microbiology
Volume 2017, Article ID 8012104, 7 pages
https://doi.org/10.1155/2017/8012104
Research Article

Characterization of Carbapenem-Resistant Acinetobacter baumannii Strains Isolated from Hospitalized Patients in Palestine

1Caritas Baby Hospital, Bethlehem, State of Palestine
2Bethlehem University, Bethlehem, State of Palestine
3Alia Hospital, Hebron, State of Palestine
4Hussein Hospital, Bethlehem, State of Palestine
5Rafidia Hospital, Nablus, State of Palestine
6Palestinian Forum for Medical Research, Ramallah, State of Palestine

Correspondence should be addressed to Musa Hindiyeh; gro.liam-brc@heyidnih.asuom

Received 25 March 2017; Accepted 18 June 2017; Published 26 July 2017

Academic Editor: Dulal Borthakur

Copyright © 2017 Regeen Handal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The American Centers for Disease Control and Prevention (CDC) recognizes Acinetobacter baumannii as a source of global outbreaks and epidemics especially due to its increasing resistance to commercially available antibiotics. In this study, 69 single patient multidrug resistant isolates collected from all over Palestine, except Gaza, were studied. All the isolates were resistant to all the β–lactam antibiotics including the carbapenems. Of the 69 isolates, 82.6% were positive for , 14.5% were positive for , and 3% were positive for . None were positive for and . In addition, 5.8% and 0% were positive for and , respectively. Of the 69 isolates, none were positive for the aminoglycoside aphA6 gene while 93% were positive for the aphA1 gene. The acetyltransferases aacC1 and aacA4 genes tested positive in 22% and 13% of the isolates, respectively. The ompA biofilm-producing virulence gene was detected in all isolates. Finally, Multilocus Sequence Typing (MLST) of 13 isolates revealed that more than one strain of A. baumannii was circulating in Palestinian hospitals as results revealed that 7 isolates were of ST208, 2 isolates ST218, 1 isolate ST231, 1 isolate ST348, and 2 new Sequence Types. The detection of these drug resistant pathogens is a reminder of the importance of active surveillance for resistant bacteria in order to prevent their spread in hospital settings.