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International Journal of Microbiology
Volume 2017 (2017), Article ID 8486742, 7 pages
https://doi.org/10.1155/2017/8486742
Research Article

Molecular Characteristics and Antibiotic Resistance Profiles of Klebsiella Isolates in Mthatha, Eastern Cape Province, South Africa

1Division of Medical Microbiology, Department of Laboratory Medicine and Pathology, Faculty of Health Sciences, Walter Sisulu University, Private Bag X1, Mthatha, Eastern Cape 5117, South Africa
2Academic Affairs, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
3Department of Biological Sciences, University of Botswana, Mobutu Drive, P.O. Box 80462, Gaborone, Botswana
4Department of Microbiology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria

Correspondence should be addressed to Sandeep Vasaikar; moc.oohay@rakiasavpeednas

Received 15 July 2016; Accepted 27 December 2016; Published 30 January 2017

Academic Editor: Barbara H. Iglewski

Copyright © 2017 Sandeep Vasaikar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The increase in the incidence of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella species has become a serious problem worldwide, because of their incrimination in antibiotic resistance. The objective of this study is to investigate the resistance genes responsible for ESBL-producing Klebsiella species and carbapenemase-producing Klebsiella (CRE) isolated in Mthatha and to study their epidemiology. A prospective, descriptive study of 202 nonrepetitive samples from patients was obtained from Nelson Mandela Academic Hospital. The cultured Klebsiella isolates were subjected to antimicrobial susceptibility tests and the polymerase chain reaction of , , , , and genes. Overall K. pneumoniae were the majority with 169 (83.7%) species isolates, followed by K. oxytoca with 29 (14.4%), while K. ozaenae and Raoultella ornithinolytica were 2 (0.9%) each. The prevalence of ESBL production in all Klebsiella species was 117 (57.9%). ESBL-genotypic resistance is driven in Mthatha by 121 (77.1%) followed by 105 (66.9%) and at 89 (56.7%). The most common ESBL genotype combination among the Klebsiella was + at 79 (50.3%). There is a steady increase in the rate of ESBL genes in the last five years.