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International Journal of Microbiology
Volume 2017 (2017), Article ID 9532170, 8 pages
https://doi.org/10.1155/2017/9532170
Research Article

Prevalence of Virulence Genes Associated with Diarrheagenic Pathotypes of Escherichia coli Isolates from Water, Sediment, Fish, and Crab in Aby Lagoon, Côte d’Ivoire

1Department of Biochemistry and Food Sciences, University of Peleforo Gon Coulibaly, BP 1328, Korhogo, Côte d’Ivoire
2Laboratory of Microbiology, Oceanographic Research Center, BP V 18, Abidjan, Côte d’Ivoire
3Laboratory of Biotechnology and Food Microbiology (UFR/STA), University of Nangui Abrogoua, 02 BP 801 Abidjan, Côte d’Ivoire

Correspondence should be addressed to Ollo Kambire

Received 17 January 2017; Accepted 27 April 2017; Published 6 June 2017

Academic Editor: Marcel H. Zwietering

Copyright © 2017 Ollo Kambire et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 19 from sediment, and 1 from crab) were positive for virulence genes, including 34 positive for elt (ETEC), 46 positive for est (ETEC), 24 positive for both elt and est, 6 positive for stx (EHEC), 1 positive for both stx + est, and 1 positive for both stx + elt. Genes eae (EPEC) and AggR (EAEC) were not detected. Nine serogroups (O114, O127, O55, O111, O86, O119, O126, O128, and O142) were identified. This study revealed the presence of diarrheagenic and nondiarrheagenic E. coli and potential public health risks if fishery products are not appropriately cooked.