Research Article

Enterococcus faecium TIR-Domain Genes Are Part of a Gene Cluster Which Promotes Bacterial Survival in Blood

Figure 5

Both tirEs are part of one operon within E1162. (a) Graphical representation of the tirE operon within E1162 and primer locations. The ORFs in the immediate surrounding of tirEs are shown by thick arrows, while thin grey and black arrows indicate the location and orientation of the primers used for RT-PCR. Amplification is indicated as a straight line, and crossed lines indicate absence of products. Grey lines and arrows illustrate junction primers amplifying intergenic regions and their products. Black lines and arrows show the internal primers and their products, which were used to confirm linkage of the gene expression. (b) RT-PCR products linking tirE1, hp1, and tirE2. RT-PCR after amplification with tirE1 internal (TirE1-F) plus hp1 internal (Hp-R) primers yielded a 777 bp band and with hp1 internal (Hp-F) plus tirE2 internal (TirE2-R) gave a 1286 bp product. −RT shows corresponding negative controls and gDNA positive controls.