Prevalence of Multidrug Resistance and Extended-Spectrum β-Lactamase Carriage of Clinical Uropathogenic Escherichia coli Isolates in Riyadh, Saudi Arabia

Table 2

Multiplex PCR-specific primers used for ESBL gene detection in Enterobacteriacaea.

PCR name	β-lactamase targeted	Sequence (5′– 3′)	Product (bp)	Reference
Multiplex I	TEM variants including TEM-1 and TEM-2	CATTTCCGTGTCGCCCTTATTC	800	[32]
		CGTTCATCCATAGTTGCCTGAC
	SHV variants including SHV-1	AGCCGCTTGAGCAAATTAAAC	713
		ATCCCGCAGATAAATCACCAC
	OXA-1, OXA-4, and OXA-30	GGCACCAGATTCAACTTTCAAG	564
		GACCCCAAGTTTCCTGTAAGTG
Multiplex II	Variants of CTX-M group 1 including CTX-M-1, CTX-M-3, and CTX-M-15	TTAGGAARTGTGCCGCTGYA	688
		CGATATCGTTGGTGGTRCCAT
	Variants of CTX-M group 2 including CTX-M-2	CGTTAACGGCACGATGAC	404
		CGATATCGTTGGTGGTRCCAT
	Variants of CTX-M group 9 including CTX-M-9 and CTX-M-14	TCAAGCCTGCCGATCTGGT	561
		TGATTCTCGCCGCTGAAG