Characterization of Erythromycin and Tetracycline Resistance in Lactobacillus fermentum Strains

Table 1

Primers used in PCR amplifications.

Target gene	Primer sequence (5′-3′)	Ta (^°C)	Amplicon size (bp)	Reference
erm(A)	F: AAGCGGTAAACCCCTCTGAG	52	441	[28]
	R: TCAAAGCCTGTCGGAATTGG
erm(B)	ermB1-F: CATTTAACGACGAAACTGGC	60	425	[28]
	ermB1-R: GGAACATCTGTGGTATGGCG
	ermB2-F: GAAAAGGTACTCAACCAAATA	59	639	[29]
	ermB2-R: AGTAACGGTACTTAAATTGTTTAC
erm(C)	F: ATCTTTGAAATCGGCTCAGG	49	295	[28]
	R: CAAACCCGTATTCCACGATT
erm(T)	F: TATTATTGAGATTGGTTCAGGG	55	395	[28]
	R: GGATGAAAGTATTCTCTAGGGATTT
mef(A)	F: CTATGACAGCCTCAATGCG	52	1400	[30]
	R: ACCGATTCTATCAGCAAAG
Int	F: GCGTGATTGTATCTCACT	50	1028	[31]
	R: GACGCTCCTGTTGCTTCT
Tet-int	F: CGGATAGATAAAGTACGATA	52	2659	[32]
	R: TCACGTCTTTTTTCTGACAT
tet(M)	tetM1-F: GAACTCGAACAAGAGGAAAGC	60	740	[32]
	tetM1-R: ATG GAAGCCCAGAAAGGAT
	tetM2-F: GGTGAACATCATAGACACGC	58	401	[33]
	tetM2-R: CTTGTTCGAGTTCCAATGC
tet(L)	tetL1-F: GTMGTTGCGCGCTATATTCC	55	696	[31]
	tetL1-R: GTGAAMGRWAGCCCACCTAA
	tetL2-F: GTTTCGGGTCGGTAATTGGG	45	220	[28]
	tetL2-R: GCTATCATTCCACCAATCGC
tet(K)	tetK1-F: TTATGGTGGTTGTAGCTAGAAA	55	348	[31]
	tetK1-R: AAAGGGTTAGAAACTCTTGAAA
	tetK2-F: GTAGCGACAATAGGTAATAG	46	278	[28]
	tetK2-R: GCAACTTCTTCTTCAGAAAG
tet(S)	F: GGAGTACAGTCACAAACTCG	55	335	[28]
	R: GGATATAAGGAGCAACTTTG
tet(W)	F: GAGAGCCTGCTATATGCCAGC	55	168	[28]
	R: GGGCGTATCCACAATGTTAAC
16S rRNA	27F: GAGTTTGATCCTGGCTCAG	51	1400	[34]
	1392R: ACGGTTACCTTGTTACGACTT